Hippocampal EEG power, activity and temperature data of control and Cav3.2 deficient mice from long-term and post-urethane recordings

Eight Cav3.2+/+ mice (mean age: 124 ± 1 days, all ♂) and eight Cav3.2-/- animals (mean age: 129 ± 4 days, all ♂) were anesthetized using ketamine / xylazine (100/10 mg/kg i.p.) and implanted with a radiofrequency transmitter (TA10ETA-F20, DSI, USA). For experimental details see Weiergräber M. et al. (2005), Lundt A. et al. (2016) and Papazoglou A. et al. (2016). Electroencephalographic recordings were performed using a radiotelemetry set-up (DSI, USA). The hippocampal EEG data from the CA1 region were obtained using deep epoxylite coated tungsten electrodes. EEGs were sampled at a nominal sampling rate of 1000 Hz. Recordings were carried out for two 24 h long-term recordings (R1, R2) with 12 h/12 h light cycle (LC)/ dark cycle (DC)). In addition, two urethane (800 mg/kg i.p.) injections (U1, U2) were performed to selectively induce type II theta activity. All recordings were carried out under non-restraining conditions in ventilated cabinets (under controlled environmental parameters). Data were Fast-Fourier Transformation (FFT) based analysed for relative power (%) of the different frequency bands including delta 1 (0.5-4 Hz), delta 2 (1-4 Hz), theta 1 (4-8 Hz), theta 2 (4-12 Hz), alpha (8-12 Hz), sigma (12-16 Hz), beta 1 (12-30 Hz), beta 2 (16-24 Hz), beta 3 (16-30 Hz), gamma low (30-50 Hz), gamma mid (50-70 Hz), gamma high (70-100 Hz), gamma ripples (80-200 Hz), and gamma fast ripples (200-500 Hz). Relative power values were provided for 2 s epochs. The FFT based approach was carried out via a DSI specific software (Neuroscore, DSI, USA). Activity data represent relative values (see Neuroscore, DSI, USA). Temperature values are provided in °C. As the original Neuroscore raw data file format requires the related software, which is not freely available, the relative power data (%) were exported together with temperature and relative activity values as Excel (.xls) files (see Data section). Ethics Statement All animal experiments were carried out in accordance with the guidelines of the German council on animal care and experimental protocols were approved by the local institutional and national committee on animal care (State Agency for Nature, Environment and Consumer Protection; Landesamt für Natur, Umwelt und Verbraucherschutz, LANUV, Germany, AZ-Nr. 87-51.04.2010.A321). All animal experimentation was further conducted in line with the ARRIVE guidelines, the National Institute of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 8023, revised 1978) revised 1996 or the UK Animals (Scientific Procedures) Act 1986 and associated guidelines, or the European Communities Council Directive of 24th November 1986 (86/609/EEC) and September 22nd, 2010 (2010/63/EU). Specific effort was made to reduce the number of experimental animals and their suffering (3R strategy).

本研究纳入8只Cav3.2+/+小鼠（平均年龄124±1天，均为雄性）与8只Cav3.2-/-小鼠（平均年龄129±4天，均为雄性），以氯胺酮/甲苯噻嗪（100/10 mg/kg，腹腔注射）进行麻醉，并植入射频发射器（TA10ETA-F20，DSI公司，美国）。实验细节参见Weiergräber M.等（2005）、Lundt A.等（2016）及Papazoglou A.等（2016）的相关研究。采用无线遥测系统（DSI公司，美国）进行脑电图（electroencephalogram, EEG）记录，使用涂覆深度环氧漆的钨电极采集海马CA1区的脑电数据，采样标称速率为1000 Hz。本次记录包含两次24小时长期记录（R1、R2），采用12小时光照/12小时黑暗的光周期（LC/DC）；此外还实施两次氨基甲酸乙酯（800 mg/kg，腹腔注射）注射（U1、U2），以选择性诱导II型θ节律活动。所有记录均在通风柜内无束缚条件下开展，环境参数处于严格受控状态。本研究采用快速傅里叶变换（Fast-Fourier Transformation, FFT）对不同频段的相对功率（%）进行分析，涵盖频段包括：δ1（0.5~4 Hz）、δ2（1~4 Hz）、θ1（4~8 Hz）、θ2（4~12 Hz）、α（8~12 Hz）、σ（12~16 Hz）、β1（12~30 Hz）、β2（16~24 Hz）、β3（16~30 Hz）、低γ（30~50 Hz）、中γ（50~70 Hz）、高γ（70~100 Hz）、γ涟漪（80~200 Hz）及快速γ涟漪（200~500 Hz）。相对功率值以2秒时长的时段（epoch）为单位提供，本次FFT分析通过DSI专用软件（Neuroscore，DSI公司，美国）完成。活动数据为相对值（详见Neuroscore，DSI公司，美国说明），温度单位为摄氏度（℃）。由于原始Neuroscore原生数据文件格式需要专用软件（非免费获取），研究将相对功率数据与温度、相对活动数据一同导出为Excel（.xls）文件（详见数据章节）。伦理声明：所有动物实验均遵循德国动物护理指南，实验方案已获当地机构及国家动物护理委员会（德国自然、环境与消费者保护局，Landesamt für Natur, Umwelt und Verbraucherschutz, LANUV，审批号AZ-Nr. 87-51.04.2010.A321）批准。所有动物实验同时符合ARRIVE指南、《美国国立卫生研究院（National Institute of Health, NIH）实验动物护理与使用指南》（NIH出版物第8023号，1978年版，1996年修订）、1986年《英国动物（科学程序）法案》及相关指南，或1986年11月24日欧盟委员会指令（86/609/EEC）及2010年9月22日欧盟委员会指令（2010/63/EU）。研究严格践行3R原则，尽力减少实验动物使用量并减轻其遭受的痛苦。