A set of tissue differentially expressed Zfp genes with TS-DMS in their 3′ terminal exon.

1) The level of methylation in the 3′exon of each Zfp gene was calculated for each tissue by adding the averaged digestion frequencies of triplicates at each CpG identified in the exon, and dividing by the number of identified CpGs in the exon. A digestion frequency around 26 is typically found in un-methylated CGI (Figure S2E in Supporting Information S1), where df2) The numbers represent the raw signal for the density of mapped reads (wiggle format) in the CSHL Long RNA-seq track in the UCSC genome browser (NCBI37/mm9 assembly).3) All 5′ CGI were found un-methylated in all the tissues for each Zfp gene listed in this table.4) Fold changes calculated as the ratio between df at testes and the avg df of the three somatic tissues.5) Fold change is calculated as the ratio of somatic tissue vs testes. The somatic tissue with the highest RNA-seq signal or “df” was chose in each case. Abbreviation: CGI for CpG Island, df for digestion frequency, B for brain, K for kidney, L for liver and T for testes.