Total and cholera toxin B-tetramer specific T cells from Peyer's patches and mesenteric lymph nodes

T cells were separately isolated from MLN and PP from two naive mice, and T cells binding to a cholera toxin B subunit specific MHC II tetramer from mice orally immunized with cholera toxin 7 days or boosted 5 days with a second dose of cholera toxin. Cells were purified using flow cytometric cell sorting and 3' (n=5) or 5' (n=5) libraries were constructed using the 10X workflow. From 5' libraries TCR libraries were also constructed and sequenced.