Data From: Astrocytic LRRK2 Controls Synaptic Connectivity via Regulation of ERM phosphorylation

Updated Supplemental Information Figure S1: Phospho-ERM is upregulated in LRRK2 G2019Ski/ki astrocytes in ACC and MOp but not SSp and DMS. Related to Figure 1. Figure S2: phospho-ERM is eliminated after Lambda protein phosphatase (λ-PPase) treatment. Related to Figure 1. Figure S3: phospho-ERM is significantly increased and colocalizes with S100β and MAP2. Related to Figure 1. Figure S4: ERM and GFAP levels are not altered in LRRK2 G2019Ski/ki ACC and MOp. Related to Figure 1. Figure S5: LRRK2 G2019S does not change excitatory synapse numbers in the primary motor cortex. Related to Figure 2. Figure S6: Overexpression of AAV-HA-tagged-WT Ezrin and AAV-HA-tagged-Phospho-dead Ezrin in WT and LRRK2 G2019Ski/ki astrocytes. Related to Figure 4. Figure S7: Overexpression of AAV-HA-tagged-WT Ezrin and AAV-HA-tagged-Phospho-dead Ezrin in WT and LRRK2 G2019Ski/ki astrocytes. Related to Figure 4. Figure S8: WT Ezrin overexpression does not change excitatory synapse numbers in the ACC of WT mice. Related to Figure 4. Figure S9: Overexpression of AAV-HA-tagged-WT Ezrin and AAV-HA-tagged-Phospho-dead Ezrin in WT and LRRK2 G2019Ski/ki astrocytes. Related to Figure 4. Figure S10: LRRK2 G2019S does not change ALDH1L1+/SOX9+ cell numbers in the ACC and MOp. Related to Figure 5. Figure S11: Localization and expression of LRRK2 in astrocytes. Related to Figure 5. Figure S12: BioID constructs expression and biotinylation in vivo and in vitro. Related to Figure 6. Figure S13: LRRK2 G2019S changes the composition of cytoplasmic proteins in astrocytes in vivo. Related to Figure 6. Figure S14: Astrocytic Atg7 is essential astrocyte morphological complexity in vitro. Related to Figure 7.