Integrated systems immunology approach identifies impaired effector T cell memory responses as a feature of progression to severe dengue fever

Typical symptoms of uncomplicated dengue fever (DF) include headache, muscle pains, rash, cough, and vomiting. A proportion of cases progress to severe dengue hemorrhagic fever (DHF), associated with vascular permeability, thrombocytopenia, and hemorrhages. Progression to severe dengue is difficult to diagnose at the onset of fever, which complicates patient triage, posing a socio-economic burden on health systems. To identify parameters associated with protection and susceptibility to DHF, we pursued a systems immunology approach integrating plasma chemokine profiling, high-dimensional mass cytometry and peripheral blood mononuclear cell transcriptomic analysis at the onset of fever in a prospective study conducted in Indonesia. RNA-seq analysis was performed using bulk PBMCs from study participants with either DF (n=5) or DHF (n=6). >>>Submitter states that raw data are unavailable due to patient privacy concerns<<<