Data from: Polycaprolactone nanofibers loaded with 20(S)-Protopanaxadiol for in vitro and vivo antitumor activity study

In this work, 20(S)-Protopanaxadiol (PPD) loaded polycaprolactone (PCL) nanofibers were successfully fabricated by electrospinning technique using tween-80 as a solubilizer. Firstly, smooth and continuous nanofibers were collected by using suitable solvent and appropriate spinning conditions. Secondly, nanofiber mats were characterized by scanning electron microscope (SEM), thermogravimetric (TG) analysis, Fourier transform infrared spectroscopy (FTIR) and mechanical test. Finally, nanofibrous membranes were evaluated using water contact angle, in vitro drug release, biodegradation test, in vitro and vivo antitumor activity and cell apoptosis assay. As a result, scanning electron microscopy observations indicated that diameter of the drug-loaded nanofibers increased with drug concentration increasing. Thermogravimetric (TG) analysis and mechanical test showed nanofibers equipped with great thermal and mechanical properties. Biodegradation test exhibited that the structure of fabricated nanofibers had a certain degree of change after 15 days. In vitro release study showed that PPD from drug-loaded nanofibers could be released in a sustained and prolonged mode. The cytotoxic effect of drug-loaded nanofiber mats examined on the human laryngeal carcinoma cells (Hep-2 cells) demonstrated that the prepared nanofibers had a remarkable antitumor effect. Meanwhile, the drug-loaded fiber mats showed a super antitumor effect in vivo antitumor study. All in all, PCL nanofibers could be a potential carrier of PPD for cancer treatment.

本研究以吐温-80为增溶剂，采用静电纺丝技术成功制备了负载20(S)-原人参二醇（20(S)-Protopanaxadiol, PPD）的聚己内酯（polycaprolactone, PCL）纳米纤维。首先，通过选用适宜溶剂与优化纺丝工艺参数，成功收集得到形貌光滑连续的纳米纤维。随后，采用扫描电子显微镜（scanning electron microscope, SEM）、热重（thermogravimetric, TG）分析、傅里叶变换红外光谱（Fourier transform infrared spectroscopy, FTIR）以及力学性能测试，对纳米纤维膜进行了系统表征。最后，通过接触角测试、体外释药实验、生物降解实验、体内外抗肿瘤活性检测与细胞凋亡实验，对纳米纤维膜的综合性能进行了评价。研究结果表明：扫描电子显微镜观测显示，载药纳米纤维的直径随药物浓度升高而增大；热重分析与力学测试证实，所制备的纳米纤维具备优异的热稳定性能与力学性能；生物降解实验显示，所制纳米纤维的微观结构在15天后出现了一定程度的变化；体外释药研究表明，载药纳米纤维中的PPD可实现持续缓释的给药模式；以人喉癌细胞（human laryngeal carcinoma cells, Hep-2细胞）为靶细胞开展的细胞毒性实验证实，所制备的纳米纤维具有显著的抗肿瘤效果；同时，体内抗肿瘤实验结果显示，载药纤维膜展现出极佳的体内抗肿瘤活性。总而言之，PCL纳米纤维有望成为PPD用于癌症治疗的潜在递送载体。