Response of Wheat Root Microbial Network, Core Microbes, and Assembly Mechanisms under Salt-Alkaline Gradients

This study set up four treatments: no stress (CK), salt stress alone (S, 100 mmol Na+), moderate salt-alkaline stress (SA1, 100 mmol Na+ + pH 8.0), and severe salt-alkaline stress (SA2, 100 mmol Na+ + pH 9.0). A pot experiment system was used to analyze the diversity, community structure, co-occurrence network, and assembly mechanisms of wheat root-associated bacterial and fungal communities under different salt-alkaline levels.