Comparative effects of selective enzyme inhibition on 125I-Ang-(1–12) metabolism in medium of cultured neonatal wky and shr cardiac myocytes.

Values are expressed as % (Mean ± SE) of Ang-(1–12) unmetabolized* and Ang peptides (%) generated from 125I-Ang-(1–12) in the cultured medium incubated for 60 min at 37°C to WKY or SHR myocytes with or without the presence of RAS inhibitors. No inhibitors group: Only aminopeptidases inhibitors (amastatin & bestatin) and carboxypeptidases inhibitor (benzyl succinate); All inhibitors group: Aminopeptidases and carboxypeptidases inhibitors + RAS inhibitors (lisinopril, SCH39370, MLN-4760 & chymostatin); Minus RAS inhibitor groups: One of the RAS inhibitor (lisinopril/SCH39370/MLN-4760/chymostatin) omitted at a time from all inhibitors group. All values are expressed as (%) and are the average of three or more independent assays.*Percent of 125IAng-(1–12) parent control remained un-metabolized in the medium exposed to WKY or SHR myocytes for 60 min at 37°C.aSignificantly different (PNo RAS inhibitors group vs. corresponding WKY or SHR group of +All RAS inhibitors.bSignificantly different (PAll RAS inhibitors.cSignificantly different (P