Data for: Enhancement of host infectivity, immunity, and protective efficacy by addition of sodium bicarbonate antacid to oral vaccine formulation of live attenuated Salmonella secreting Brucella antigens

The present study investigates the importance administration of sodium bicarbonate for the gastric survival and immune enhancement of oral Brucella vaccine prepared from attenuated Salmonella secreting Brucella antigens SodC and Omp19 in BALB/c mice. Mice were immunized with PBS, Salmonella alone or with antacid treatment. The antacid treatment was given 30 minutes before and immediately after immunization with the attenuated Salmonella. Employing green fluorescent protein-expressing attenuated Salmonella strain and immunohistochemical assay showed a significant intensity of GFP expression and Salmonella invasion in ileum regions of the intestinal epithelium. The humoral and cell-mediated immune responses were evaluated on each mice group and our results showed significant induction of IgG antibody response, splenocyte proliferative response and CD4+ T cell subpopulation in antacid treated mice group. Upon virulent Brucella challenge, a significant reduction of Brucella colonization and inflammation in spleen and liver tissues were observed in antacid treated mice group than Salmonella alone or PBS control group.

本研究探讨了碳酸氢钠给药对于由分泌布鲁氏菌抗原SodC与Omp19的减毒沙门氏菌制备的口服布鲁氏菌疫苗在BALB/c小鼠中实现胃部存活与免疫增强的重要作用。实验小鼠分别经磷酸盐缓冲液（Phosphate Buffered Saline, PBS）、单独减毒沙门氏菌或联合抗酸剂处理进行免疫，抗酸剂给药方案为在减毒沙门氏菌免疫前30分钟及免疫后即刻进行。借助表达绿色荧光蛋白（GFP）的减毒沙门氏菌菌株及免疫组化分析，可观察到肠道上皮回肠区域的GFP表达信号与沙门氏菌侵袭程度均显著增强。对各组小鼠的体液免疫与细胞介导免疫应答进行评估后发现，抗酸剂处理组小鼠的IgG抗体应答、脾细胞增殖应答以及CD4+ T细胞亚群均得到显著诱导。经强毒布鲁氏菌攻击后，与单独减毒沙门氏菌组及PBS对照组相比，抗酸剂处理组小鼠的脾脏与肝脏组织中布鲁氏菌定植量与炎症反应均显著降低。