Metagenomic analysis in a mouse model of steatohepatitis

It is previously established that the ratio of Bacteroidetes and Firmicutes, the two dominant phyla that make up over 90% of bacterial phylogenetic types in the human distal gut, is modulated by the diet; in particular a diet enriched in fat causes a modulation of the intestinal microbiota composition that results in a decreased proportion of Bacteroidetes which is compensated by a robust increase in the proportion of Firmicutes. The goals of this study are to confirme these findings analyzing the composition of microbiota in mice fed a High Fat diet and fructose (HFD-F), and to evaluate the effects of two bile acid receptors ligands, Ursodeoxycholic acid (UDCA) and Obeticholic acid (OCA), on intestinal flora in this mouse model of NASH. Here we report the information obtained from metagenomics workflow for each sample.

此前已有研究证实，拟杆菌门（Bacteroidetes）与厚壁菌门（Firmicutes）这两个占人类远端肠道细菌系统发育类群90%以上的优势菌门，其比例受饮食调控；具体而言，富含脂肪的饮食可改变肠道菌群组成，使拟杆菌门比例降低，同时伴随厚壁菌门比例显著升高以代偿该变化。本研究旨在通过分析喂食高脂果糖饮食（High Fat Diet and Fructose, HFD-F）的小鼠肠道菌群组成，验证上述发现，并评估两种胆汁酸受体配体——熊去氧胆酸（Ursodeoxycholic acid, UDCA）与奥贝胆酸（Obeticholic acid, OCA）——对该非酒精性脂肪性肝炎（NASH）小鼠模型肠道菌群的影响。本文报道了从每个样本的宏基因组学（metagenomics）分析流程中获得的相关信息。