Viral fitness landscapes in diverse host species reveal multiple evolutionary lines for the NS1 gene of influenza A viruses - Supplemental Data (Part 1)

We designed a barcoded viral library of recombinant influenza A viruses expressing a selection of NS1 from different strains, hosts, countries and years. Each virus was individually rescued and deep-sequenced using the Illumina MiSeq platform to verify viral genome integrity and sequence. This dataset includes Illumina deep-sequencing raw data of each virus included in the final library (barcodes 1-123).

本研究构建了一款携带条形码标记的重组甲型流感病毒文库，该文库可表达来自不同毒株、宿主、国家及年份的多种非结构蛋白1（NS1）。每株病毒均经独立拯救获得，并采用Illumina MiSeq平台开展深度测序，以验证病毒基因组的完整性与序列准确性。本数据集包含最终文库中所有病毒（条形码编号1-123）的Illumina深度测序原始数据。