ETV2 regulates PARP-1 binding protein to induce ER stress-mediated cell death in tuberin-deficient cells

Syk inhibitor demonstrates antiproliferative effect on Tsc2-deficient cells and LAM lung nodules similar to rapamycin. A feedback loop between Syk inhibition and mTORC1 inhibition pathways is also present in these Tsc2-deficient cells. We used microarrays to discern the potential difference in Syk inhibition and mTORC1-inhibition pathways. The goal was to investigate regulatory pathways or targets of Syk inhibition to induce cytocidal response in Tsc2-deficient cells, independent of its crosstalk with mTORC1 signaling. Tsc2-deficient ELT3V cells were plated in 10 cm culture dish per condition in DMEM culture media supplemented with 10% FBS. Twenty-four hours after plating cells were starved overnight (16 hours). The cells were then treated with DMSO, Syk-inhibitor or rapamycin for 24 hours. Cells were harvested by trypsinization for total RNA isolation and microarray processing.