Genomewide profiling of differentially H3K4me3 marked genes in colon cancer cells versus normal colon epithelial cells.. Genomewide profiling of differentially H3K4me3 marked genes in colon cancer cells versus normal colon epithelial cells.

To identify differentially marked genes with a narrow or broad H3K4me3 pattern, we performed ChIP-seq using formaldehyde crosslinked chromatin from three colorectal cancer cell lines and normal colonic epithelial cell line and was digested with micrococcal nuclease to the extent that 70% of the chromatin was cleaved to mononucleosomes. Overall design: To explore the differentially marked genes with a narrow or broad H3K4me3 pattern, we performed ChIP-seq using formaldehyde crosslinked three colorectal cancer cell lines and normal colonic epithelial cells.