Next Generation Sequencing of HUVECs stimulated by conidia of Aspergillus fumigatus

Purpose: The goal of this study is to investigate the responses of HUVECs after the stimulation of conidia of A. fumigatus Methods: HUVECs were stimulated with conidia of Aspergillus fumigatus for 2 and 6 hours. Three biological repeats of stimulated cells or un-stimulated controls were send for RNA sequencing. Results: Using an optimized data analysis workflow, we mapped about 40 million sequence reads per sample to the human genome (build hg38) and identified round 80,000 transcripts in the HUVECs upon stimulation. Conclusions: Our resutls showed the detailed analysis of HUVECs transcriptomes upton conidia of Aspergillus fumigatus stimulation. HUVEC cells were seeded in 6-well-plate (2x10^6 cells per well) overnight before the conidia of A. fumigatus were added to the wells at a cells:spores ratio of 1:2. The cell lysates were harvest 2 and 6 hours post stimulation.