G1 Phase Shortening via Chir-124 Treatment in HEK293 Cells

The goal of this experiment was to test the potential of the Chk1 inhibitor Chir-124 to accelerate HEK293 through the G1 phase of their cell cycle. Cells were treated with Chir-124 for 48 hours before being harvested and stained with propidium iodide for determination of percentage of the cell population in each cell cycle phase.
Conclusion:
Analysis of HEK293 cells following Chir-124 treatment for 48 hours shows substantial loss of cells in the G1 phase, instead showing more even distributions between G1, S, and G2/M phases. Additionally, there is a population of PI stained cells with less fluorescence than the G1 population that may indicate mirconuclei forming with treatment. Unstained samples were used to calibrate the flow cytometer prior to experimental run