nanoLC‑MALDI‑TOF/TOF Proteomic Profiling of SARS‑CoV‑2 Spike Pseudovirus‑Infected hACE2 Cells Treated with Honeybee (Apis mellifera) Venom, Apamin, and Tertiapin

This dataset contains raw and processed mass spectrometry data generated for the study: “Honeybee (Apis mellifera) venom and its bioactive peptides tertiapin and apamin modulate the proteome of SARS‑CoV‑2‑infected cells.” The aim of the study was to characterize host proteome remodeling in human ACE2‑expressing HEK293 (hACE2) cells infected with SARS‑CoV‑2 spike‑pseudotyped lentiviral particles and treated with: Whole honeybee venom (HBV) Apamin (APA) Tertiapin (TERT) Proteomic profiling was performed using nano‑liquid chromatography coupled to MALDI‑TOF/TOF tandem mass spectrometry (nanoLC‑MALDI‑TOF/TOF MS/MS). Experimental Design hACE2 cells were: Infected with SARS‑CoV‑2 spike pseudotyped lentiviral particles (MOI 1.33) Treated with: HBV (1 µg/mL and 2 µg/mL) Apamin (0.5, 1, 2 µg/mL) Tertiapin (0.25, 0.5, 1, 2 µg/mL) Compared to infected untreated control cells Each condition was analyzed in: Two biological replicates Two technical replicates Mass Spectrometry Workflow Protein extraction: TAP buffer with protease and phosphatase inhibitors Digestion: Trypsin (in-solution digestion, modified Pierce protocol) Peptide cleanup: ZipTip C18 nanoLC system: Easy‑nLC II (Bruker Daltonics) Fractionation: Proteineer‑fc II fraction collector Matrix: HCCA (α‑cyano‑4‑hydroxycinnamic acid) Target: AnchorChip 384 (Bruker Daltonics) Instrument: UltrafleXtreme MALDI‑TOF/TOF (Bruker Daltonics) Acquisition mode: Reflectron mode MS/MS analysis: TOF/TOF fragmentation Raw Data Files (RAW) The repository includes: Complete Bruker MALDI‑TOF/TOF raw data folders (.mcf format) All nanoLC fractions (384 spots per sample) Raw files were acquired using: FlexControl 3.4 FlexAnalysis 3.4 These files preserve the original vendor format required for reanalysis. Identification / Result Files (RESULT) Protein identification was performed using: Software: Mascot v2.4.1 Database: NCBInr Taxonomy: Homo sapiens The dataset includes: Mascot search result files Protein identification lists Organism Homo sapiens (human HEK293 ACE2-expressing cells) Instrument Bruker UltrafleXtreme MALDI‑TOF/TOF mass spectrometer