Nano genome altas (NGA) of body wide tissue specific responses

Purpose: In order to detect the unpredictable risks of the cumulative nanoparticles infiltrated everywhere in the whole body after systematic administration Methods: By using RNA-seq, we systematically studied the transcriptomic response of multi-organs from mice and tissue specific (stem) cells to the hydroxyapatite (HA) nano-delivery system. Results: We show that HA could differentially influence gene expression, immune response, cell proliferation, stress, apoptosis, metabolic, ion transport activity, cell signaling and epigenetic activity across tissues and tissue specific (stem) cells. Overall design: We collected mouse tissues of heart, liver, spleen, kidney, skeletal muscle, peripheral blood mononuclear cells (PBMC) as well as tissue specific (stem) cells of mouse skeletal muscle derived stem cells (MUSC), Human bone marrow derived stem cells (hBMSC), Human primary umbilical vein endothelial cell lines (HUVEC) for RNA-seq after treated with the two groups (PBS control and HA solution) for 24hours, respectively.