Metagenome-assembled genomes from Slate River floodplain sediments near Crested Butte, CO, USA (September 2019)

Microorganisms play a key role in cycling nutrients and contaminants in the terrestrial environment depending on their genetic potential. Here we present metagenome-assembled genomes (MAGs) for the bacterial and archaeal community in floodplain sediment samples taken September 2019 at one locations (OBJ1) near the confluence of the Oh-Be-Joyful Creek and Slate River. The site is one of the field sites in focus for the Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory Groundwater Quality Science Focus Area (SFA) program. Sediment samples from a deep soil pit were collected from 50 to 150 cm depth below surface at discrete depths every 20 cm for microbial analyses. A total of 6 metagenomes were sequenced through the Joint Genome Institute (JGI) and can be found under Genomes Online Database (GOLD) sequencing project: Gs0142591. Metagenomes were assembled, binned, and refined using metawrap to generate MAGs (>50% complete and < 10% contamination based on checkM scores). This dataset includes a zip file of 2562 MAG fasta files and a csv file with quality, taxonomic classification (Genome Taxonomy Database Release RS220), and metagenome accessions for MAGs. This dataset also includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type. Part of this work was performed at SLAC Accelerator Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-76SF00515.

微生物凭借自身的遗传潜能，在陆地环境的营养物质与污染物循环过程中发挥关键作用。本研究针对2019年9月采集于Oh-Be-Joyful溪与斯莱特河（Slate River）汇流点附近一处点位（OBJ1）的河漫滩沉积物样本中的细菌与古菌群落，构建并发布了宏基因组组装基因组（metagenome-assembled genomes, MAGs）。该点位是斯坦福直线加速器中心（Stanford Linear Accelerator Center, SLAC）国家加速器实验室地下水质量科学重点区域（Science Focus Area, SFA）项目的重点野外采样点位之一。研究人员从一处深土坑的地表下50至150 cm深度区间，按每20 cm的间隔分层采集沉积物样本，用于微生物组分析。研究团队通过联合基因组研究所（Joint Genome Institute, JGI）完成了总计6个宏基因组的测序，相关数据可在基因组在线数据库（Genomes Online Database, GOLD）的测序项目Gs0142591中获取。本研究使用Metawrap工具对宏基因组进行组装、分箱与优化，最终得到符合CheckM评分标准的MAGs（完整度>50%且污染率<10%）。本数据集包含一个包含2562个MAG FASTA文件的压缩包，以及一个记录MAG质量、分类学注释（基于基因组分类数据库Genome Taxonomy Database Release RS220）与宏基因组登录号的CSV文件。本数据集还包含一个文件级元数据文件（flmd.csv），用于列出数据集中所有文件及其关联元数据；以及一个数据字典文件（dd.csv），其中收录了所有文件中使用的行列表头，及其对应的定义、单位与数据类型。本研究部分工作在SLAC加速器实验室完成，由美国能源部科学办公室生物与环境研究局通过合同号DE-AC02-76SF00515资助。