Global proteome analysis.
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https://figshare.com/articles/dataset/Global_proteome_analysis_/21265722
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HT29 cells were cultured in the presence or absence of Sgg strain TX20005 for 24 hours. The cells were washed, lysed, and digested with Trypsin then analyzed by a nanoLC-1200 system coupled to an Orbitrap Fusion Lumos mass spectrometer. Obtained spectra were searched against the target-decoy Human RefSeq database (release 2020) in Proteome Discoverer 2.1 interface (PD 2.1, Thermo Fisher) with the Mascot algorithm (Mascot 2.4, Matrix Science). Protein inference and quantitation were performed by gpGrouper (v1.0.040) with shared peptide iBAQ area distribution (Saltzman et al 2018 PMID 30093420) then convert to iFOT. iFOT is the normalization of individual protein intensity to the total protein intensity within one experiment.
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创建时间：
2022-10-03
