Rank Correlation.

Spearman's rank correlations were calculated between the variables of PCONS, Z-score, SENS, DIST, and %Mask. See Materials and Methods for the definition of Spearman's rank correlation. %Mask is from Table 2. Z-Score and SENS are from Table 5. DIST is from the first row of Table S2. PCONS is from the bottom line of Table S1. Pcons: probability of residue conservation, Z-score: average Z-score 602 active sites, SENS: sensitivity, DIST: distance between two ESSTs, %Mask: percentage of discarded functional residues.