A Library Construction Method for Single-Cell Genome that Carries Randomly Inserted Barcodes

A central challenge in sequencing single-cell genome is to determine point mutations, phasing of these mutations, and copy number variations without any presumptions. We report a new library construction method which has a potential to meet this goal. In such library, every fragment has a unique barcode (UFI for unique fragment identifier) that identify the fragment itself and its two immediate neighbors. Having this wealth of information, shallow sequencing of the library can be used to determine copy number variations much more reliably than with extant methods