Loss of Plrx function does not impair Plasmodium life cycle progression

aPercentage of mosquitoes that contain oocysts at day 11–13 post-feeding.bMidgut-associated sporozoites per infected mosquito at day 12–14 post-feeding.cSalivary gland-associated sporozoites per infected mosquito at day 17–19 post-feeding.dLiver stages are mature exo-erythrocytic forms visualized 48 hours after incubation of 10,000 salivary gland sporozoites with subconfluent cultured hepatocytes.ePrepatent period is the time until the first detection of an erythrocytic stage parasite in Giemsa-stained blood smears after intravenous injection of 10,000 salivary gland sporozoites (i.v.) or by bites of 5–10 infected mosquitoes.fFour independent Plrx(-) clones (# 1,2,5, and 7) were fed. Shown are the average values from these phenotypically indistinguishable clones.N/D, not done.