PIKfyve controls dendritic cell function and tumor immunity I

To understand the mechanism of Pikfyve loss in dendritic cells, we cultured conventional dendritic cells isolated from the bone marrow of Pikfyvef/f (“WT”) and mice with ItgaxTg/0 Pikfyvef/f (“KO”) mice and performed transcriptome profiling on RNA extracted from cells on Day 9 in culture. Taken together, these data demonstrate the PIKfyve mediates suppression of DC transcriptional maturation programs through the alternate/non-canonical NF-κB regulatory pathway. Comparative gene expression profiling of RNA-seq data from primary cultured conventional dendritic cells isolated from wild-type (WT) or Pikfyve-deficient (KO) mice (littermate pair-matched biological triplicates).