RNA-sequencing of melanoma celllines compared to normal human melanocytes (NHEMs)

Given that melanomas gain different properties during their progression, 6 different melanoma cell lines (Sbcl-2, WM3211, WM793, WM1366, WM1158, WM9) were selected and categorized by tumor stage (primary tumor (PT), metastasis (MET)) as well as the previously described BRAF and PTEN mutation status. Then, differentially expressed genes in malignant melanoma were identified by performing RNA-Sequencing (RNA-seq) with all aforementioned melanoma cell lines and normal human epidermal melanocytes (NHEM). Total RNA-Seq of the melanoma celllines Sbcl-2, WM3211, WM13166, WM793, WM1158, WM9 and normal human epidermal melanocytes (NHEM).