视网膜测序数据

获取胚胎发育期第12，14周的视网膜组织，将组织剪碎成小块，用木瓜蛋白酶和胶原酶IV以及DNase1将其消化成单细胞悬液，终止消化反应后进行流式分选，获取活性较好的视网膜单细胞，利用10x genomics对单细胞悬液进行扩增建库，获取单细胞文库，文库质检合格后送至测序公司使用Illumina平台测序，测序长度为双端150bp。

Retinal tissues at embryonic developmental stages of 12 and 14 weeks were collected. The tissues were minced into small fragments, then digested into a single-cell suspension using papain, collagenase IV and DNase I. After terminating the digestion reaction, flow cytometry sorting was performed to obtain retinal single cells with good viability. The single-cell suspension was amplified and library constructed using 10x Genomics to acquire single-cell libraries. After passing the quality inspection of the libraries, the qualified samples were sent to a sequencing company for sequencing on the Illumina platform, with a read length of paired-end 150 bp.

搜集汇总

背景与挑战

背景概述

该数据集包含人类胚胎第12和14周视网膜组织的单细胞测序数据，使用10x基因组学和Illumina平台进行测序，旨在研究视网膜细胞的命运和神经回路机制。数据由中国科学院生物物理研究所的王晓群团队提供，遵循CC BY 4.0许可协议。