Effect of anti-IL-4 and anti-IL-13 vaccination in a mouse model of atopic dermatitis

The effect of anti-mouse IL-4 and/or anti-mouse IL-13 Kinoid vaccines was assessed in a model of atopic dermatitis. Mice were immunized with IL-4 and/or IL13 Kinoids (or the carrier protein CRM197 alone as control). Atopic dermatitis-like pathology was induced by repeated epicutaneous exposures to house dust mite and the enterotoxin B from Staphylococcus aureus (SEB). Lesional skin biopsies were collected for bulk RNA sequencing analysis. Overall design: Bulk RNAseq on back skin of vehicle or HDM-SEB-treated mice (immunized with Kinoids® or control) at D56 (end of the model) (n = 30 samples). RNA extraction was performed using RNeasy Lipid Tissue Kit (Qiagen). Libraries were produced using the llumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (Illumina), according to the manufacturer's instructions. RNA sequencing was performed on one S4 lane of the Illumina NovaSeq 6000 instrument (Illumina, San Diego, CA, USA), using the NovaSeq 6000 S4 v1.5 Reagent Kit (300 cycles) and a paired-end 2 ×150 pb strategy. Raw reads were processed with the nf-core/rnaseq workflow v3.12.0 using Nextflow v22.12.0-edge. Analyses on raw counts are performed on R v4.2.2. The DESeq2 package (v1.38.3) was used to normalize data and run the differential expression analyses. Differentially expressed genes were defined as genes with an adjusted p-value below 0.05 and an absolute log2 Fold Change above 1.