RNA-Seq analysis of classical monocyte in pateints with sickle cell disease [RNA-Seq]

Patients with sickle cell disease (SCD) suffer from intravascular hemolysis associated vascular injury and tissue damage. Classical monocytes (CMo), which comprise most blood circulating monocytes. are activated in SCD, but the cause and consequences of activation is less clear. Using transcriptome analysis, we found upregulation of the type I Interferon (IFN) pathway in sort-purified SCD CMo. Overall design: CD14+CD16- classical monocyte were purifed with CD14 microbeads and sorted with flow cytometer