Next generation sequencing facilitates quantitative analysis of pAd-KLF5- and pAd-Control-infected mouse VSMC transcriptomes

Purpous: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathway. The goals of this study are to compare different gene expression between pAd-KLF5- and pAd-Control-infected mouse VSMC. Methods: adenovirus vectors either encoding KLF5 or empty adenovirus vector were transfected into mouse VSMCs. shSirt6 transfected or shControl transfected VSMCs samples were performed RNA sequencing (RNA-seq) analysis, functional enrichment analyses of gene ontology (GO) and gene set enrichment analysis (GSEA). Results: eIF5a is found to be highly expressed and predictive target of KLF5 in VSMCs. KLF5-enhanced VSMCs demonstrated cell proliferation and were noted to have promoted cell cycle progression between the G2 and M phases. In accordance with the gene set enrichment analysis (GSEA) according to RNA-sequencing, ATP biosynthesis and cell redox homeostasis was upregulated upon KLF5 overexpression. Conclusion: These findings provide mechanistic insights into the oncogenic activity of KLF5 for the first time. Overall design: RNA profiles of test and control samples were generated by deep sequencing, using Illumina HiSeq 4000.