Transcription factor cooperation and competition

Transcription factors have to distinguish proper targets from a large pool of potential binding sites. Here we explore how MSL2 and CLAMP cooperate to bind their specific targets. Using an in vitro chromatin reconstitution system and by mutating the interaction domain of CLAMP we show how direct and indirect cooperation of TF binding influence binding specificity. ChiP-seq profiles of MSL2 CLAMP and CLAMP mutant in vitro reconstituted chromatin