Quantification of four arsenic species in fruit juices by ion-chromatography–inductively coupled plasma–mass spectrometry

A method using ion chromatography–inductively coupled plasma–mass spectrometry (IC–ICP–MS) for the quantification of arsenic species in fruit juices has been developed and validated. The method is capable of quantifying four anionic arsenic species – arsenite (As(III)), arsenate (As(V)), dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) – in the presence of unretained species such as arsenobetaine (AsB). Method validation was based on repeatability, analysis of reference materials, recovery of fortified samples, and determination of detection and quantification limits. The method was tested for use with apple, pear, cranberry, grape (red, white and purple) juices, as well as several juice blends. Limits of detection were 0.35, 0.41, 0.45 and 0.70 µg kg−1 for As(III), DMA, MMA and As(V), respectively. Chromatographic recovery was good for most samples (90–107% compared to total arsenic), though recovery for some grape juice samples was lower (67–78%).

本研究开发并验证了一种采用离子色谱-电感耦合等离子体质谱法（ion chromatography–inductively coupled plasma–mass spectrometry，IC–ICP–MS）定量果汁中砷形态的分析方法。该方法可在砷甜菜碱（arsenobetaine，AsB）等不保留组分共存的条件下，对四种阴离子砷形态进行定量：亚砷酸盐（arsenite，As(III)）、砷酸盐（arsenate，As(V)）、二甲基次胂酸（dimethylarsinic acid，DMA）与一甲基胂酸（monomethylarsonic acid，MMA）。方法验证基于重复性实验、标准参考物质分析、加标样品回收率测定以及检出限与定量限的确定。本方法针对苹果汁、梨汁、蔓越莓汁、红/白/紫葡萄汁以及多款混合果汁开展了适用性测试。四种砷形态的检出限分别为As(III) 0.35 μg·kg⁻¹、DMA 0.41 μg·kg⁻¹、MMA 0.45 μg·kg⁻¹及As(V) 0.70 μg·kg⁻¹。多数样品的色谱回收率良好（与总砷含量相比为90%~107%），但部分葡萄汁样品的回收率偏低，为67%~78%。