Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Zfyve19-/- hepatic Transcriptomes

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived hepatic transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis Overall design: hepatic mRNA profiles of 6-8 weeks wild type (WT) and Zfyve19-/- mice