Genome sequencing and assembly for 20 wild isolates

This study aims to understand the intraspecific genomic divergence of the fission yeast Schizosaccharomyces pombe by generating genome assemblies from PacBio long-read sequencing data. A total of 20 S. pombe strains were sequenced using PacBio RSII or Sequel platform. Their genomes were assembled using three different long-read assemblers (Canu, Raven, and Flye). A total of 60 assemblies were generated, with three assemblies for each strain. The assemblies were polished using both PacBio sequencing reads and Illumina sequencing reads.