Molecular characterisation of faecal bacterial assemblages among four species of syntopic odonates

Factors such as host species, phylogeny, diet, timing, and location of sampling are thought to influence the composition of gut-associated bacteria in insects. In this study, we compared the faecal-associated bacterial taxa for three Coenagrion and one Enallagma damselfly species. We expected high overlap in representation of bacterial taxa due to the shared ecology and diet of these species. Using metabarcoding based on the 16S rRNA gene, we identified 1513 sequence variants, representing distinct bacterial ‘taxa’. Intriguingly, the damselfly species showed somewhat different magnitudes of richness of ZOTUs, ranging from 480 to 914 ZOTUs. In total, 921 (or 60.8% of the 1513) distinct ZOTUs were non-shared, each found only in one species, and then most often in only a single individual. There was a surfeit of these non-shared incidental ZOTUs in the Enallagma species accounting for it showing the highest bacterial richness and accounting for a sample-wide pattern of more single-spec..., To assess the faecal bacterial assemblages of damselflies, we targeted four predatory odonate species at a freshwater pond of approximately 600 m × 200 m (12 ha), located in Southern Finland (ETRS-TM35FIN N: 67118; E: 2460). On 1–2 June 2016, we collected 185 individuals (20–26 males and females from each species) for faecal DNA analysis. All our focal damselfly species belong to the family Coenagrionidae: Coenagrion lunulatum (Charpentier, 1840), Coenagrion hastulatum (Charpentier, 1825), Coenagrion pulchellum (Vander Linden, 1825), and Enallagma cyathigerum (Charpentier, 1840). Species identification of damselflies was based on current literature, e.g. [27]. These four target species were selected as they were the most common predatory species at the study site, based on pilot surveys (K. Kaunisto, pers. obs.). Only sexually mature individuals with adult colours and hardened wings were included in the study. According to a previous study [28], all four focal species feed mainly on dip..., , # Data from: Molecular characterisation of faecal bacterial assemblages among four species of syntopic odonates

[https://doi.org/10.5061/dryad.08kprr58q](https://doi.org/10.5061/dryad.08kprr58q)

Molecular data from faecal PCR-based 16S sRNA DNA analysis from four damselfly species.

## Description of the data and file structure

DNA extraction using Macherey-Nagel NucleoSpin Tissue XS Kit (product nr 740901, Macherey-Nagel, Düren, Germany), followed by 16S gene PCR using primers 515F-Parada 5′-GTG YCA GCM GCC GCG GTA A-3′ (Parada et al. 2016) and 806R-Apprill 5′-GGA CTA CNV GGG TWT CTA AT-3′ (Apprill et al. 2015), and library preparation. Sequencing was carried on Illumina MiSeq v3 2x300bp run.

Data consists of raw FASTQ files with two technical replicates per sample. Retrieved sequence variants (ZOTUs), ZOTU tables, and ZOTU assignations are also included. See the original article for more detailed information.

### File contents and Column names

##### File = rdp\_16s\_v18\_plus\_...

宿主物种、系统发育关系、食性、采样时间与采样地点等因素，被认为会影响昆虫肠道共生细菌的群落组成。本研究针对3种蓝豆娘属（Coenagrion）与1种亮翅豆娘属（Enallagma）的豆娘物种，比较了其粪便相关细菌分类单元。我们推测，由于这些物种共享生态与食性特征，其细菌分类单元的组成将存在较高重叠。本研究基于16S rRNA基因的宏条形码测序（metabarcoding），共鉴定出1513个序列变异型，代表不同的细菌“分类单元”。有趣的是，4种豆娘的零半径操作分类单元（ZOTU, Zero-radius Operational Taxonomic Unit）丰富度存在一定差异，其范围为480至914个ZOTU。总计有921个独特ZOTU（占总1513个序列变异型的60.8%）为非共享类群，即仅在单个物种中被发现，且其中多数仅存在于单一个体中。亮翅豆娘物种拥有大量这类非共享的偶发ZOTU，这也是其细菌群落丰富度最高的原因，同时呈现出单个体特有类群占比更高的样本级模式……

为评估豆娘的粪便细菌群落，我们在芬兰南部一处面积约600米×200米（12公顷）的淡水池塘（ETRS-TM35FIN坐标系：N: 67118; E: 2460）中选取了4种掠食性蜻蛉目物种。2016年6月1日至2日，我们采集了185个个体（每个物种包含20~26只雄性与雌性个体）用于粪便DNA分析。所有目标豆娘物种均属于蟌科（Coenagrionidae）：新月蓝豆娘（Coenagrion lunulatum, Charpentier, 1840）、矛斑蓝豆娘（Coenagrion hastulatum, Charpentier, 1825）、美丽蓝豆娘（Coenagrion pulchellum, Vander Linden, 1825）以及普通亮翅豆娘（Enallagma cyathigerum, Charpentier, 1840）。豆娘的物种鉴定依据现有文献，例如[27]。基于预调查结果（K. Kaunisto, 个人通讯），我们选择了研究区域内最常见的4种掠食性蜻蛉目物种作为研究对象。本研究仅纳入具有成虫体色与硬化翅膀的性成熟个体。根据此前一项研究[28]，这4种目标物种主要以双翅目昆虫为食……

# 数据来自：四种同域分布蜻蛉类群粪便细菌群落的分子表征
[https://doi.org/10.5061/dryad.08kprr58q]

本数据集包含基于粪便PCR扩增的16S rRNA DNA分析得到的4种豆娘的分子数据。

## 数据与文件结构说明
DNA提取采用Macherey-Nagel NucleoSpin Tissue XS试剂盒（产品编号740901，Macherey-Nagel，德国迪伦），随后使用引物515F-Parada 5′-GTG YCA GCM GCC GCG GTA A-3′（Parada等，2016）与806R-Apprill 5′-GGA CTA CNV GGG TWT CTA AT-3′（Apprill等，2015）进行16S基因PCR扩增，并完成文库制备。测序采用Illumina MiSeq v3 2×300bp测序平台。
数据集包含每个样本带有两个技术重复的原始FASTQ文件，同时还包含已获取的序列变异型（ZOTU）、ZOTU丰度表以及ZOTU分类注释结果。详细信息请参阅原文。

### 文件内容与列名
##### 文件 = rdp_16s_v18_plus_...