RNA-seq analysis CFPAC-1-siNC and CFPAC-1-siUBE2C

The transcriptome sequencing results were used to determine the association between UBE2C and pancreatic cancer. First, the RNA-seq data revealed that the expression of 2332 genes were different in the siUBE2C group compared with the negative control group. There were 363 genes and 152 genes with two-fold increased and decreased expression, respectively. The top 30 GO terms enriched among all differentially expressed genes included biological processes (attachment of spindle microtubules to kinetochore, regulation of attachment of spindle microtubules to kinetochore, protein localization to chromosome), cellular components (condensed nuclear chromosome, condensed chromosome outer kinetochore) and molecular functions (single-stranded DNA-dependent ATPase activity, intestinal epithelial cell maturation). The major enriched pathways were the cell cycle pathway and the p53 signaling pathway. Overall design: After transfecting CFPAC-1 cells with siUBE2C for 36 hours, total RNA was extracted and performed RNA-seq, contained three siUBE2C-treated samples and three matched control samples(siNC).