Specialized regulatory T cells control venous blood clot resolution through SPARC

The cells and mechanisms involved in blood clot resorption are only partially known. Regulatory T cells (Treg) accumulate in venous blood clots and regulate thrombolysis by controlling the recruitment, differentiation and matrix metalloproteinase (MMP) activity of monocytes. The clot Treg population is heterogeneous and contains a population of Treg that forms the matricellular acid- and cysteine-rich protein (SPARC). SPARC induces MMP activity in monocytes and SPARC+ Treg are required for clot resorption. Whole transcriptome analysis (WTA) of CD25+ single cells from day 12 thrombi and vein walls of thrombotic B6 mice (n=10, pooled) The linked python script can be used to translate cell indexes to raw sequences found in the fastq files. See the linked readme file for further instructions.