Resistance to SYN351 generated by ectopic expression of VAChT in D. melanogaster.

Resistance factors are ratios of LD50 values obtained by fitting a regression analysis to the relationship between % mortality and log (dose); parallel regression was accepted as the model for all the data. All genotypes are described in the online methods.Resistance factors for ectopic expression of the wild-type vacht transgene in two independent lines, as compared to the parental genotypes (Cha and vacht+(1−n)).Resistance factors for ectopic expression of the vachtY49N transgene in three independent lines, as compared to the parental genotypes. Since no mortality was observed at the highest dose tested (1 mg.ml−1), resistance factors are the minimum expected based on estimates of LD50 that assume parallel dose response curves to those observed in (i).(i) Resistance factors for ectopic expression of the vachtE341K transgene in three independent lines, as compared to the parental genotypes. No significant resistance was detected in the test genotypes compared to both of the parental controls.