Primary cilia, transition zone protein B9d1 is required for proper cell growth and ribosome biogenesis during meiotic prophase in males

To investigate how primary cilia or ciliary signaling pathway(s) influence gene expression profile during meiotic prophase in male flies, we used RNA-seq from testes of B9d1 Knock-down, Tektin-C Knock-down, Klp59D Knock down, wild-type and sa-/- mutant. Overall design: Each sequencing assay included spermatocyte-enriched samples and wild-type samples, with 2 replicates for each condition