H.pylori 26695 WT / AGS

Tilt Series Date: 2014-03-31 Data Taken By: Yi-Wei Chang Species / Specimen: Helicobacter pylori Strain: 26695 Tilt Series Settings: Single Axis, tilt range: (-65.0°, 65.0°), step: 1.5°, constant angular increment, dosage: 130.0 eV/Ų, defocus: -10.0 μm, magnification: 22500x. Microscope: Caltech Polara Acquisition Software: Undefined Upload Method: pipeline Processing Software Used: Raptor Collaborators and Roles: H.pylori cells from Carrie Shaffer Purification / Growth Conditions / Treatment: H.pylori cells grown in BLBB + 5% heat inactivated fetal bovine serum at 37 degrees in 5% CO2 chamber for O/N, OD600=~1.3. Back dilute to OD 0.3, grew to OD 0.6 for infection. AGS cells cultivated on QF Au finder grid for 1 day in RPMI + 10% FBS. Infect by pylori for 4 hr. Sample Preparation: Vitrobot settings: 100% humidity, 37 C, 3µl BSA treated 20 nm gold (100 ul gold+ 25 ul BSA, pellets from 8 tubes merged into ~40 ul final solution). Manual blot by Whatman 40 filter paper for 3 sec. Ethane/propane mix. ycw2014-03-31-21_slicer9186.jpg: Helicobacter pylori . extracellular vesicle ycw2014-03-31-21_slicer9187.jpg: Helicobacter pylori . REZ ribosome exclusion zone ycw2014-03-31-21_slicer17878.jpg: cag T4SS wi fi particle (partially assembled?)