Gallic Acid Pretreatment Mitigates Parathyroid Ischemia-Reperfusion Injury through Signaling Pathway Modulation

As a polyphenolic herbal extract, Gallic acid (GA) is known for its antioxidant and anti-inflammatory properties. However, its application in parathyroid ischemia-reperfusion injury (IRI) has not been well examined. This study aimed to elucidate the protective effects of GA pretreatment in a parathyroid IRI model and explore the underlying mechanisms.Hypoxia and hypoxia/reoxygenation models were established with primary parathyroid cells to explore the protective effect of GA.The results from sequencing and bioinformatic analyses indicated that GA might regulate MMP1, MMP9, FOSB, GSY2, DUSP5, TRPV1/3, TIAM1 and multiple signaling pathways in this process such as IL-17, AMPK, MAPK, Transient Receptor Potential (TRP) channels, cAMP and Rap1. Overall design: mRNA-seq was collected from control untreated parathyroid cells (Negative Control group, NC), hypoxia/reoxygenation cells (IRI (6H-6H) cell (Model group, M)), and GA-pretreated hypoxia/reoxygenation cells (IRI (6H-6H) cells that were pretreated with1200 uM GA (Treatment group, T) ) to unravel the potential underlying mechanism.