Metagenomic Whole Genome Shotgun Analysis of the Airway Microbiome in Laryngotracheal Stenosis: A Pilot study

The airway microbiome has been implicated in the pathogenesis of laryngotracheal stenosis (LTS), yet studies using amplicon-based 16S rRNA sequencing are limited to sub-genus level resolution. Metagenomic whole genome shotgun sequencing (mWGS) allows for strain-level taxonomic and functional genomic analysis, providing detailed insights into the specific organisms and pathways involved. A pilot study was conducted to explore the feasibility, advantages, and challenges of mWGS in investigating the airway metagenome in LTS