Phloroglucinol derivatives from <i>Hypericum</i> species induce <i>in vitro</i> proliferation of cells involved in the wound healing process

The genus <i>Hypericum</i> (Hypericaceae) is a recognized source of therapeutic agents, being some species widely used due to their wound healing properties. In a previous study, south Brazilian species <i>H. caprifoliatum, H. carinatum, H. connatum, H. myrianthum</i> and <i>H. polyanthemum</i> demonstrated potential to induce proliferation of keratinocytes. In the present study, the effect of phloroglucinol derivatives isolated from <i>Hypericum</i> on cell proliferation of human keratinocytes, fibroblasts and stem cells was investigated. The best results, determined by the MTT assay, were achieved with cariphenone B at concentrations of 0.01 and 0.1 µM (122.3% and 114%, respectively) on HaCaT cells. Uliginosin B was able to induce the proliferation of mesenchymal stem cells (129% at 10 µM) and MRC5 fibroblasts (152.5% at 5 µM). These findings confirm the capacity of phloroglucinol derivatives to induce the <i>in vitro</i> cellular proliferation and reinforce the importance of <i>Hypericum</i> species as potential sources of wound healing compounds.

金丝桃属（*Hypericum*，金丝桃科Hypericaceae）是公认的药用活性成分来源，其中部分物种因其促伤口愈合特性而被广泛应用。此前一项研究表明，巴西南部的短柱金丝桃（*H. caprifoliatum*）、具肋金丝桃（*H. carinatum*）、连萼金丝桃（*H. connatum*）、多花金丝桃（*H. myrianthum*）以及多腺金丝桃（*H. polyanthemum*）均具备诱导角质形成细胞增殖的潜力。本研究针对从金丝桃属植物中分离得到的间苯三酚类衍生物（phloroglucinol derivatives）对人角质形成细胞、成纤维细胞及干细胞增殖的影响展开了探究。通过MTT检测（MTT assay）确定，在HaCaT细胞中，卡里菲诺酮B（cariphenone B）在0.01 µM和0.1 µM浓度下可取得最优增殖效果，增殖率分别达122.3%与114%。乌里金诺辛B（Uliginosin B）则可诱导间充质干细胞（mesenchymal stem cells）增殖（10 µM浓度下增殖率为129%），同时对MRC5成纤维细胞亦有促增殖作用（5 µM浓度下增殖率达152.5%）。上述研究结果证实了间苯三酚类衍生物具备诱导体外细胞增殖的能力，同时进一步凸显了金丝桃属植物作为促伤口愈合活性成分潜在来源的重要价值。