Proteins, source and dilution of antibodies, staining procedures and patterns and interpretation analysis.
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DAB: 3,3′-Diaminobenzidine; C: Cytoplasmic; EGFR: epidermal growth factor receptor; ENZ: Proteolytic enzyme; ER1: Citric acid, pH 6.0;ER2: ethylenediaminetetraacetate, pH 8.8; HP: Hot plate; HRP: Horseradish peroxidase; M: Membranous; N: Nuclear; SQ: semi-quantitative.(1): Leica Biosystems, Newcastle, UK; (2): Dako, Glostrup, Denmark; (3): Invitrogen, Carlsbad, CA.1Scores 1+, 2+ and 3+ were considered to be positive; 2Score 3+ in >30% of tumor cells was considered to be positive.*Double IHC was performed for the following antibodies: CK5/p53, CK14/Ki67 and CK17/Vimentin in the TMAs of the HE10/97 trial.
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2015-12-02
