Differential gene expression analysis identified determinants of cell fate plasticity during radiation-induced regeneration in Drosophila

We used here a combination of lineage tracing, FACS and genome-wide RNAseq to identify two key changes that accompany hinge-to-pouch fate change: (1) repression of hinge determinants and (2) upregulation of ribosome biogenesis. Overall design: We performed a genome-wide analysis of gene expression changes during regeneration of the Drosophila larval wing disc. By dissociating the discs into single cells and using Fluorescence-Activated Cell Sorting we monitored the hinge versus the rest of the disc, to identify genes that are differentially expressed in the two cell populations during regeneration and fate change.