Single-cell transcriptome analysis reveals a cell subpopulation involved in vascular endothelial growth factor secretion of human mesenchymal stem cells under ischemia

Using scRNA-seq analysis on human mesenchymal stem cells (MSCs), we identified a functional cell subpopulation positively correlated with the VEGF secretion under ischemia. Our data indicates that a strategy based on single-cell transcriptome profiles may be effective for identifying functional cell subpopulations in heterogeneous MSCs. Overall design: Single-cell RNA-seq analyses of MSCs from many donors