File S1 - Arginine 199 and Leucine 208 Have Key Roles in the Control of Adenosine A2A Receptor Signalling Function

Supporting Figures and Tables. Figure S1, NECA-induced activity of the WT A2AR, Rag23 and the quadruple mutants intermediate between the WT and Rag23. See Table 1 for the precise details of each mutant. The receptor constructs were expressed in the MMY24 S. cerevisiae strain using the p306GPD vector. The activity of cells containing empty vector is shown as a control. Figure S2, NECA-induced activity of the WT A2AR and the triple mutants intermediate between the WT and Rag23. See Table 1 for the precise details of each mutant. The receptor constructs were expressed in the MMY24 S. cerevisiae strain using the p306GPD vector. The activity of cells containing empty vector is shown as a control. Figure S3, NECA-induced activity of the WT A2AR and the double mutants intermediate between the WT and Rag23. See Table 1 for the precise details of each mutant. The receptor constructs were expressed in the MMY24 S. cerevisiae strain using the p306GPD vector. The activity of cells containing empty vector is shown as a control. Figure S4, NECA-induced activity of the WT A2AR and the single mutants intermediate between the WT and Rag23. See Table 1 for the precise details of each mutant. The receptor constructs were expressed in the MMY24 S. cerevisiae strain using the p306GPD vector. The activity of cells containing empty vector is shown as a control. Table S1, Oligos used to generate the mutant receptor constructs. Table S2, Expression levels of the thirty mutants and the wild-type calculated using the eGFP fluorescence as described by Drew et al. (2008, Nature Protocols, 3: 784–798). (DOCX)