Data for: Triose phosphate utilization stress during photosynthesis addressed with dynamic assimilation measurements

Oscillations in CO2 assimilation rate and associated fluorescence
parameters have been observed alongside the triose phosphate utilization
(TPU) limitation of photosynthesis for nearly 50 years. However, the
mechanics of these oscillations are poorly understood. Here we utilize the
recently developed Dynamic Assimilation Techniques (DAT) for measuring the
rate of CO2 assimilation to increase our understanding of what
physiological condition is required to cause oscillations. We found that
TPU limiting conditions alone were insufficient, and that plants must
enter TPU limitation quickly to cause oscillations. We found that ramps of
CO2 caused oscillations proportional in strength to the speed of the ramp,
and that ramps induce oscillations with worse outcomes than oscillations
induced by step change of CO2 concentration. An initial overshoot is
caused due to a temporary excess of available phosphate. During the
overshoot, the plant out-performs steady state TPU and ribulose
1,5-bisphosphate regeneration limitations of photosynthesis but cannot
exceed the rubisco limitation. We performed additional optical
measurements which support the role of photosystem I reduction and
oscillations in availability of NADP+ and ATP in supporting oscillations.