Chemotactic response of hESCs to conditioned medium from first trimester villous explant cultures.

Decidualized hESCs were subjected to transwell migration assay using TCM, or supernatants from three individual first trimester villous explant cultures (VECM), as the chemoattractant (undiluted). The control medium for TCM treatment was MM1-10%, that for VECM was serum-free medium incubated with collagen as detailed in Materials & Methods (VECM-Co). Motility indices represent means±SD (n = 3) and were analyzed by ANOVA and Tukey test. ***, P