Comparing the transcription level between fourth day and eighth day in Streptomyces avermitilis S0

Streptomyces avermitilis is a avermectin producer.Since the avermectin biosynthesis rate has a increased significantly in P3 fermentation stage( P1,24–96 h; P2:96–192 h, P3:192–240 h), but the sugar absorption rate decreased significantly in P3 fermentation stage, in order to improve the titer of avermectins, we conducted transcriptomic analysis of Streptomyces avermitilis S0 in fourth and eighth day, and selected native promoters with appropriate temple using to express sugar transporters. We chose two time points in fourth and eighth day. The samples were collected at each time point named S0-4d, S0-8d.