CIL:12957, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein; larger gold particles) and GOLPH3 (a trans-Golgi associated protein that can also be associated with mitochondria; smaller gold particles) staining. Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.

本数据集针对泌乳大鼠的乳腺组织开展免疫电子显微镜观察，旨在探究成熟脂滴与反式高尔基体网络（trans-Golgi network）各组分间潜在的结构相互作用。实验采用TGN38（一种跨膜反式高尔基体蛋白，对应较大金颗粒）与GOLPH3（一种既可定位于反式高尔基体、也可结合线粒体的反式高尔基体相关蛋白，对应较小金颗粒）进行染色以标记靶标结构。需注意，泌乳乳腺细胞内的反式高尔基潴泡含有大量酪蛋白与其他乳蛋白，且因分泌液的渗透压作用发生显著扩张。