Physicochemical properties of Pin2 and Pin2 variants.
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GRAVY, Sequence Grand average of hydropathicity, calculated using the Expasy ProtParam tool (http://web.expasy.org/protparam/), according to Kyte and Doolittle, 1982 [44].AMF, Average Molecular Flexibility values were calculated according to Liu et al., 2008 [45].μHrel, Relative Hydrophobic Moment, a value of 0.5 thus indicates that the peptide has about 50% of the maximum possible amphipathicity. Calculated using HydroCalc (http://www.bbcm.univ.trieste.it/~tossi/HydroCalc/HydroMCalc.html).Q, Net charge.RT, Retention Time in minutes.(§)Mass Spectrometry, ESI-MS (Finnigan LCQDUO ion trap mass spectrometer, San José, CA, USA).
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2015-12-02
